Since the toolkit is written in Python, it should work perfectly on Windows.
You might need to add the installation directory of the translate toolkit to your path
path "C:\Program Files\translate-toolkit\"
This will work for one session, but will be lost when you reboot again. Therefore you might want to add it to the autoexec.bat file.
You can add to the path permanently. Check this useful guide. You should add the following to your path:
If you have the Gettext tools installed, add it to your path as well:
Some programs in Windows will add CRLFs to the file which is considered rather poor practice for l10ns that require Unix files. To fix a text file, drag and drop it to the dos2unix.exe utility from http://www.bastet.com/